Composite

Part:BBa_K4164010:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


constitutive expression of RXR-ddRFPB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1928
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 418
    Illegal AgeI site found at 1445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800


Design Notes

We recombined low intensity promoter (BBa_J23105) and RBS (BBa_B0034) and RXR-ddRFPB1(BBa_K4164997) by homologous recombination.


Source

This sequence is composed of BBa_J23105, BBa_B0034 and BBa_K416997.

References